Course Description:

ONT Sequencing Assembly and Quality Control

The ONT Sequencing Assembly and Quality Control course provides an in-depth exploration of de novo genome assembly, polishing, and quality assessment techniques tailored for Oxford Nanopore Technologies (ONT) sequencing data. Participants will learn key principles and hands-on methods for assembling long-read sequencing data, refining assemblies, and evaluating quality metrics to ensure high-confidence genomic interpretations.

This course is designed for researchers and students in genomics and bioinformatics who seek to develop proficiency in genome assembly and assessment using state-of-the-art tools. The curriculum blends theoretical instruction with hands-on experience, equipping participants with essential skills to analyze and validate sequencing data.

What You’ll Learn

• The differences between de novo genome assembly and reference-based assembly.
• How to assemble ONT sequence data using Flye.
• Techniques for polishing genome assemblies with Medaka to improve accuracy.
• Methods to assess assembly quality using QUAST and interpret key assembly metrics.
• Best practices for ensuring high-quality genome reconstruction from long-read sequencing data.

Participants will gain practical experience with user-friendly bioinformatics tools and workflows, enabling them to confidently apply these methods to their research projects.